|
| Status |
Public on Nov 23, 2022 |
| Title |
ELN.TNF_E_2 |
| Sample type |
RNA |
| |
|
| Source name |
Keratinocytes
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Keratinocytes
treatment: TNF+ELN
replicate: 2
|
| Treatment protocol |
Cells were incubated with test materials in KC maintenance medium for 24 hours.
|
| Growth protocol |
Neonatal human epidermal KCs (passage 8) were plated in KC growth medium at late subconfluence density (~220,000 cells/well).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen (Hilden, Germany) RNAeasy Mini Plus kit with QIAcube Connect automated nucleic acid purification instrument.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared following the standard Affymetrix protocol using the GeneChip WT labeling kit.
|
| |
|
| Hybridization protocol |
Microarray hybridizations were performed using standard protocols by ThermoFisher (Waltham, MA).
|
| Scan protocol |
The standard Affymetrix protocol was following using the GeneChip™ Scanner.
|
| Description |
Gene expression data from TNF+ELN-treated KCs
|
| Data processing |
Raw CEL files were normalized using the robust multichip average (RMA) algorithm to generate log2-scaled signal intensities (Irizarry et al., 2003).
|
| |
|
| Submission date |
Oct 26, 2022 |
| Last update date |
Nov 23, 2022 |
| Contact name |
William R Swindell |
| E-mail(s) |
William.Swindell@UTSouthwestern.edu
|
| Organization name |
UT Southwestern Medical Center
|
| Department |
Internal Medicine
|
| Street address |
6201 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-9175 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE216614 |
Bakuchiol and ethyl (linoleate/oleate) synergistically modulate endocannabinoid tone in keratinocytes and repress inflammatory pathway mRNAs |
|
