|
| Status |
Public on Sep 18, 2012 |
| Title |
HKCa_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Keratinocytes
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: CaCl2 treated
cell type: Keratinocytes
|
| Treatment protocol |
Human keratinocytes were treated with 1.5 mM of calcium chloride for 48 hours.
|
| Growth protocol |
IMR90 human primary lung embryo fibroblasts (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) plus 10% FBS (Hyclone), 100 U/ml penicillin (Gibco), and 100 µg/ml streptomycin (Gibco) at 37°C in 5% CO2. Growing cells with 50~70% confluence were used for further analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
~200 ng of total RNA were extracted from ~50 million cells by using Trizol (Invitrogen) according to manufacturer’s instructions, and further purified by RNeasy Plus Mini Kit (QiaGen). cDNA libraries were prepared by using SuperScript III First-strand Synthesis SuperMix with random haxamers (Invitrogen).
|
| Label |
Cy3
|
| Label protocol |
Synthesized cDNAs were amplified and labelled with Cy3 (Perkin Elmer) by using Agilent One Colour Low RNA Input Linear Amplification Labelling Kit according to manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Labelled cDNAs were hybridized to the Agilent Human Whole-genome array (G2534-60011) as per manufacturer's instructions.
|
| Scan protocol |
standard Agilent protocol
|
| Description |
biological replicate 2 of 2
|
| Data processing |
standard Agilent protocol
|
| |
|
| Submission date |
Jan 25, 2011 |
| Last update date |
Sep 18, 2012 |
| Contact name |
Jing-Yu Li |
| E-mail(s) |
jingyuli@mednet.ucla.edu
|
| Organization name |
University of California, Los Angeles
|
| Street address |
615 Charles E. Young Dr. South BSRB 357
|
| City |
Los Angeles |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (2) |
| GSE26855 |
Gene expression profile of H1 human embryonic stem cells, IMR90 lung fibroblasts, calcium induced keratinocytes, and primary hepatocytes |
| GSE26979 |
Human Linker Histone H1.5 |
|
