|
| Status |
Public on Sep 14, 2012 |
| Title |
Normal Muscle individual 2 |
| Sample type |
RNA |
| |
|
| Source name |
Muscle tissue
|
| Organism |
Homo sapiens |
| Characteristics |
individual: 2
tissue: deltoid
gender: M
disease state: Normal Muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from muscle biopsies using the Trizol reagent followed by an additional clean up step on Qiagen RNAeasy column, following protocols provided by the manufacturer
|
| Label |
biotin
|
| Label protocol |
Biotin labelled cRNA was produced using the Ambion TotalPrep RNA amplification kit
|
| |
|
| Hybridization protocol |
Hybridization was performed on an illumina air hybridization owen. Slides were washed and stained using reagents and protocols provided by Illumina.
|
| Scan protocol |
Slides were scanned on an Illumina Iscan BeadChip reader, following the protocol provided by illumina for the HumanHT-12_V3_0_R1 BeadChip.
|
| Data processing |
Data were extracted from the scan files using Illumina Genome Studio and exported both as raw and preprocessed expression data. Before analysis, the dataset was quantile normalized, local background was not subtracted, and the expression values for the missing bead were imputed using the GenomeStudio imputation algoritm
|
| |
|
| Submission date |
Jan 25, 2011 |
| Last update date |
Jan 26, 2015 |
| Contact name |
Mario Pescatori |
| E-mail(s) |
mariopescatori@gmail.com
|
| Phone |
00393930761013
|
| Organization name |
Health e-genomics
|
| Street address |
Westplein 11
|
| City |
rotterdam |
| ZIP/Postal code |
3016BM |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE26852 |
Gene expression analysis of FSHD muscle with different MRI pattern |
|
| Relations |
| Reanalyzed by |
GSE65314 |
