|
| Status |
Public on Oct 18, 2011 |
| Title |
NO4 |
| Sample type |
RNA |
| |
|
| Source name |
NO4
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: normal_tissue_from_ccRCC_patient
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol , 10 ug of Qiagen-purified total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
|
| Data processing |
The data were analyzed with MBNI re-mapped CDF files, version 9. RMA calculated intensity from MBNI custom annotation with the affy R package.
|
| |
|
| Submission date |
Jan 14, 2011 |
| Last update date |
Oct 18, 2011 |
| Contact name |
Karl Dykema |
| Organization name |
Van Andel Institute
|
| Lab |
Lab of Computational Biology
|
| Street address |
333 Bostwick Ave. NE
|
| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
| |
|
| Platform ID |
GPL11433 |
| Series (1) |
| GSE26574 |
An antioxidant response phenotype is shared between hereditary and sporadic type 2 papillary renal cell carcinoma |
|
