E14 embryos were harvested for MEFs. The treatment is as follows: "Cells were grown to confluency. 2 Days post confluency, cells were treated with 1 microM dexamethasone, 500 microM isobutylmethylxanthine, 5 microgram/milliliter insulin and 15 microM troglitazone for 2-3 days. Cells were then changed to media containing 5 micrograms/milliliter insulin and 15 microM troglitazone for 2 days. After this, cells were maintained in normal media (DMEM+10% serum) until harvest."
