|
| Status |
Public on Aug 04, 2022 |
| Title |
Methylation_EPIC_sample221 |
| Sample type |
genomic |
| |
|
| Source name |
peripheral blood leukocytes
|
| Organism |
Homo sapiens |
| Characteristics |
race: African American (AA)
gender: M
age(yrs): 37.875427789
plate: WG5125122-MSA4
column: 2
row: B
pedigreeid: pedid_370
subjectid: subjectid_1028
phase: 1
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted from stored peripheral blood leukocytes using AutoGen FlexStar (AutoGen, Holliston, MA) and DNA was bisulfite converted using the EZ DNA Methylation Kit (Zymo Research, Irvine, CA)
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to IIllumina Infinium HumanMethylationEPIC BeadChip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
NA
|
| Data processing |
For each chip, the Minfi R package was used to perform background correction and normalization. The regression on correlated probes (RCP) method was then used to adjust probe-type bias.
|
| |
|
| Submission date |
Aug 01, 2022 |
| Last update date |
Aug 04, 2022 |
| Contact name |
Lulu Shang |
| E-mail(s) |
shanglu@umich.edu
|
| Organization name |
University of Michigan
|
| Department |
Biostatistics
|
| Street address |
1415 Washington Heights
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48105 |
| Country |
USA |
| |
|
| Platform ID |
GPL21145 |
| Series (2) |
| GSE210255 |
Methylation data from stored peripheral blood leukocytes from African American participants in the GENOA study with Infinium HumanMethylationEPIC BeadChip |
| GSE210256 |
Methylation data from stored peripheral blood leukocytes from African American participants in the GENOA study |
|
