|
| Status |
Public on Jul 19, 2022 |
| Title |
LLC Lung 2 |
| Sample type |
RNA |
| |
|
| Source name |
3LL-GFP_lung_RIN 7.90
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/BL6 mice
tissue: lung
cell type: Lewis Lung Carcinoma (LLC)
condition: cell line, intrapulmonary transplantation, GFP+ cell isolation at 7 days post-transplantation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction using Qiagen's RNeasy Plus Mini Kit was performed according to the manufacturer's instructions. RNA was quantified using a NanoDrop spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
The sense cDNA was then fragmented and biotin-labeled with TdT (terminal deoxynucleotidyl transferase) using the GeneChip WT Terminal labeling kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Hybridized arrays were washed and stained on the Agilent Technologies GeneChip Fluidics Station 450 and scanned on the Agilent Technologies Scanner GCS3000.
|
| Description |
GFP-laveled 3LL (murine lung cancer cell line)
|
| Data processing |
Array data export processing and analysis was performed using Affymetrix® GeneChip Command Console® Software (AGCC)
|
| |
|
| Submission date |
Jul 18, 2022 |
| Last update date |
Jul 19, 2022 |
| Contact name |
Ryo Miyata |
| E-mail(s) |
rmiyata@kuhp.kyoto-u.ac.jp
|
| Organization name |
Kyoto University Graduate School of Medicine
|
| Department |
Thoracic Surgety
|
| Street address |
54 Kawaharacho, Shogoin, Sakyoku
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
6068507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE208373 |
Lewis Lung Carcinoma (LLC) cells in the lung model express lung-specific genes compared to subcutaneous engraftment cells. |
|
