|
| Status |
Public on Jul 13, 2012 |
| Title |
Glioblastoma_gbm-0549 |
| Sample type |
RNA |
| |
|
| Source name |
Human glioblastoma
|
| Organism |
Homo sapiens |
| Characteristics |
patient population: Chinese
tissue: brain
disease state: primary glioblastoma multiform (brain tumor)
tumor grade: WHO grade 4
patient age: 35
patient gender: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with mirVana miRNA Isolation kit (Ambion, Austin, TX) according to the manufacturer’s protocol. Quality control was performed with NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Houston, TX).
|
| Label |
Cy3
|
| Label protocol |
Standard Illumina labelling protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol with Illumina BeadReader
|
| Data processing |
The data were background subtracted and normalized using quantile normalization with Illumina BeadStudio software v3.1
|
| |
|
| Submission date |
Nov 28, 2010 |
| Last update date |
Jul 13, 2012 |
| Contact name |
Wei Zhang |
| E-mail(s) |
zhangwei_vincent@yahoo.com.cn
|
| Organization name |
Beijing Tiantan Hospital, Capital Medical University
|
| Department |
Glioma Center, Department of Neurosurgery
|
| Street address |
No.6 Tiantan Xili, Dongcheng District
|
| City |
Beijing |
| ZIP/Postal code |
100050 |
| Country |
China |
| |
|
| Platform ID |
GPL8179 |
| Series (2) |
| GSE25631 |
Genome-wide analysis of microRNA expression in surgical specimens of primary glioblastoma multiform |
| GSE25632 |
Surgical specimens of primary glioblastoma multiform: mRNA and miRNA profiling |
|
