|
Status |
Public on Nov 30, 2013 |
Title |
Eutopic endometrium_y |
Sample type |
RNA |
|
|
Source name |
Endometrium
|
Organism |
Homo sapiens |
Characteristics |
donor: patient tissue: endometrium disease state: Normal (eutopic)
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
Description |
eutopic endometrium y.CEL
|
Data processing |
Data were analysed with oneChannelGUI (1.16.2) Bioconductor (2.7) package: RMA + quantile normalization
|
|
|
Submission date |
Nov 28, 2010 |
Last update date |
May 30, 2014 |
Contact name |
Raffaele A Calogero |
E-mail(s) |
raffaele.calogero@unito.it
|
Phone |
++39 0116706454
|
Organization name |
University of Torino
|
Department |
Molecular Biotechnology Center
|
Lab |
Bioinformatics and Genomics Unit
|
Street address |
Via Nizza 52
|
City |
Torino |
State/province |
To |
ZIP/Postal code |
10126 |
Country |
Italy |
|
|
Platform ID |
GPL571 |
Series (1) |
GSE25628 |
Endometriosis transcription profiling |
|