|
| Status |
Public on Nov 12, 2010 |
| Title |
ChIP-Seq analysis of H3K36me3 in AdiposeNuclei cells; Lib:XZ:20100107:09--ChIP:XZ:20100104:07:AdiposeNuclei:H3K36Me3 |
| Sample type |
SRA |
| |
|
| Source name |
Nuclei derived from crude preps of adipose tissue; Lib:XZ:20100107:09--ChIP:XZ:20100104:07:AdiposeNuclei:H3K36Me3
|
| Organism |
Homo sapiens |
| Characteristics |
collection_method: surgery
donor_health_status: NA
molecule: genomic DNA
disease: none
tissue_type: Adipose Nuclei
passage_if_expanded: NA
donor_ethnicity: NA
donor_sex: Female
biomaterial_type: Primary Tissue
tissue_depot: abdomen
donor_id: 7
biomaterial_provider: Harvard University
donor_age: 49
chip_protocol_bead_type: Protein A-Sepharose 4B
chip_antibody: H3K36Me3
chip_protocol_chromatin_amount: 10 million cells
extraction_protocol: SDS lysis
chip_protocol_antibody_amount: 2-15 ug
chip_protocol_bead_amount: 70 ul bed volume
chip_protocol: Bernstein_BROAD_ENCODE_protocol
extraction_protocol_sonication_cycles: 1
chip_antibody_catalog: ab9050
chip_antibody_provider: Abcam
chip_antibody_lot: 499302
experiment_type: Histone H3K36me3
extraction_protocol_type_of_sonicator: Bioruptor
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Library construction protocol: Single read - Illumina
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
sample_term_id: UBERON_0014455
assay_term_id: OBI_0000716
nucleic_acid_term_id: SO_0000352
Design description: Human Chromatin IP REMC Sequencing on Illumina
Library name: Lib:XZ:20100107:09--ChIP:XZ:20100104:07:AdiposeNuclei:H3K36Me3
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.3801
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSAMPLE%2FEDACC.3891
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
BED and WIG files were replaced on April 29, 2014
|
| Data processing |
**********************************************************************
ANALYSIS FILE NAME: GSM621461_BI.Adipose_Nuclei.H3K36me3.7.bed
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: Lib:XZ:20100107:09--ChIP:XZ:20100104:07:AdiposeNuclei:H3K36Me3.hg19.level.1
ANALYSIS TITLE: Mapping of Adipose Nuclei Histone H3K36me3 ChIP-Seq Data
ANALYSIS DESCRIPTION: Illumina reads produced by Histone H3K36me3 ChIP-Seq on Adipose Nuclei, Donor 7 were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.4588
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
ALIGNMENT_POSTPROCESSING: None
READ_EXTENSION: 200bp
RELEASE_NUMBER: Human Epigenome Atlas 2
QUALITY SCORES:
NUMBER_OF_H3K36me3_EXPERIMENTS_SCORED: 54
FINDPEAKS_SCORE: 0.353
FINDPEAKS_PERCENTILE: 72
HOTSPOT_SCORE: 0.3075
HOTSPOT_PERCENTILE: 64
IROC_SCORE: 0.9942
IROC_PERCENTILE: 91
POISSON_SCORE: 0.5926
POISSON_PERCENTILE: 70
**********************************************************************
ANALYSIS FILE NAME: GSM621461_BI.Adipose_Nuclei.H3K36me3.7.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: Lib:XZ:20100107:09--ChIP:XZ:20100104:07:AdiposeNuclei:H3K36Me3.hg19.level.2
ANALYSIS TITLE: Raw Signal Density Graphs of Adipose Nuclei Histone H3K36me3 ChIP-Seq Data
ANALYSIS DESCRIPTION: Illumina Histone H3K36me3 ChIP-Seq read mappings from Adipose Nuclei, Donor 7 were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.4862
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 200bp
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
GENOMIC_WINDOW: 20bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 2
BROWSER_TRACK_NAME: AN H3K36me3 7
BROWSER_TRACK_DESCRIPTION: BI Adipose Nuclei Histone H3K36me3 Donor 7 EA Release 2
QUALITY SCORES:
NUMBER_OF_H3K36me3_EXPERIMENTS_SCORED: 54
FINDPEAKS_SCORE: 0.353
FINDPEAKS_PERCENTILE: 72
HOTSPOT_SCORE: 0.3075
HOTSPOT_PERCENTILE: 64
IROC_SCORE: 0.9942
IROC_PERCENTILE: 91
POISSON_SCORE: 0.5926
POISSON_PERCENTILE: 70
**********************************************************************
|
| |
|
| Submission date |
Nov 10, 2010 |
| Last update date |
Jan 29, 2015 |
| Contact name |
BROAD INSTITUTE |
| E-mail(s) |
rharris1@bcm.tmc.edu
|
| Organization name |
Broad Institute
|
| Street address |
-
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL9115 |
| Series (1) |
| GSE19465 |
BI Human Reference Epigenome Mapping Project: ChIP-Seq in human subject |
|
| Relations |
| BioSample |
SAMN00119959 |
| Named Annotation |
GSM621461_BI.Adipose_Nuclei.H3K36me3.7.wig.gz |
