GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM618279 Query DataSets for GSM618279
Status Public on Nov 23, 2010
Title xenopus_axons_1
Sample type RNA
Source name Axons of retinal ganglion cells
Organism Xenopus laevis
Characteristics cell type: retinal ganglion
tissue: axons of retinal ganglion cells
Growth protocol Whole eye primordia from stage 24 and 32 Xenopus embryos were cultured on polyethylene terephthalate membrane slides for 17h and 20 h respectively. Mouse retinal explants were obtained from E15 embryos and were cultured for 24 h
Extracted molecule total RNA
Extraction protocol Total RNA were extracted from laser capture microdissected samples using RNAqueous Microkit (Ambion) and were amplified using the two-cycle TargetAmp kit (Epicentre)
Label biotin
Label protocol aRNA were biotin-labelled using GeneChip IVT labeling kit (Affymetrix)
Hybridization protocol 15ug of biotin-labelled RNA were hybridized onto Xenopus Laevis or Mouse 430 2.0 arrays for 16 h at 45C. Followed by washing and staining using Affymetrix Fluidics Station FS450 according to manufacturing instructions
Scan protocol The arrays were scanned by Affymetrix GeneChip Scanner 3000 7G using AGCC Software 2.0
Description localized mRNA from the axons of retinal ganglion cells
Data processing The arrays were normalized by robust multiarray average (RMA) using Affymetrix Expression Console software.
Submission date Nov 05, 2010
Last update date Nov 23, 2010
Contact name Brian Yee Hong Lam
Organization name University of Cambridge
Department Metabolic Research Laboratories
Street address Box 289 Addenbookes Hospital, Hills Road
City Cambridge
ZIP/Postal code CB2 0QQ
Country United Kingdom
Platform ID GPL1318
Series (1)
GSE25166 Subcellular expression profiling of the growth cones of retinal ganglion cells (RGC)

Data table header descriptions
VALUE rma normalization

Data table
AFFX-BioB-5_at 6.0773
AFFX-BioB-M_at 6.07156
AFFX-BioB-3_at 5.58523
AFFX-BioC-5_at 6.84582
AFFX-BioC-3_at 7.13037
AFFX-BioDn-5_at 8.35805
AFFX-BioDn-3_at 10.1311
AFFX-CreX-5_at 11.4903
AFFX-CreX-3_at 12.3373
AFFX-DapX-5_at 2.10407
AFFX-DapX-M_at 1.97036
AFFX-DapX-3_at 2.15252
AFFX-LysX-5_at 1.97802
AFFX-LysX-M_at 3.45354
AFFX-LysX-3_at 1.98888
AFFX-PheX-5_at 2.07526
AFFX-PheX-M_at 2.08444
AFFX-PheX-3_at 4.27915
AFFX-ThrX-5_at 3.05464
AFFX-ThrX-M_at 2.33472

Total number of rows: 15611

Table truncated, full table size 374 Kbytes.

Supplementary file Size Download File type/resource
GSM618279.CEL.gz 1.9 Mb (ftp)(http) CEL
GSM618279.chp.gz 136.8 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap