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| Status |
Public on Sep 21, 2022 |
| Title |
Eluate, 100 nM |
| Sample type |
SRA |
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| Source name |
Sample 64
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| Organism |
Severe acute respiratory syndrome coronavirus 2 |
| Characteristics |
fraction: Eluate
hr1 concentration: 100
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| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA display was performed on a library of variants. Peptides were selected based on their ability to bind to a His6-tagged HR1 peptide, which was purified with Ni-NTA beads.
Peptide-mRNA fusions were reverse-transcribed using Superscript III (ThermoFisher) and oligo-dT(20-mer) as an RT primer. After precipitation, cDNA was PCR-amplified, agarose-gel purified, and sequenced.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
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| Description |
mRNA display
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| Data processing |
Alignment of paired-end FASTQ files using Kallisto (Bray et al., 2016).
Supplementary files format and content: Tab-delimited read count file, as output from the aligner.
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| Submission date |
May 17, 2022 |
| Last update date |
Sep 21, 2022 |
| Contact name |
Timothy J Eisen |
| E-mail(s) |
timeisen@berkeley.edu
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| Organization name |
UC Berkeley
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| Department |
QB3
|
| Lab |
John Kuriyan
|
| Street address |
Stanley Hall
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| City |
Berkeley |
| State/province |
CALIFORNIA |
| ZIP/Postal code |
94720 |
| Country |
USA |
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| Platform ID |
GPL28866 |
| Series (1) |
| GSE203229 |
Nanomolar inhibition of SARS-CoV-2 infection by N-terminally extended HR2 peptides |
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| Relations |
| BioSample |
SAMN28500244 |
| SRA |
SRX15317062 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM6165050_abundance_S64.tsv.gz |
171 b |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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