|
| Status |
Public on May 15, 2022 |
| Title |
total RNA (polyA selected) from MTD-Gal4 control KD biological rep1 |
| Sample type |
SRA |
| |
|
| Source name |
ovary
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: ovary
genotype: w, MTD/+; MTD/+; MTD/pW20>w_sh[attP2]/;
age: 7 days old
|
| Growth protocol |
25˚C, 12h dark/light
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was extracted using Trizol
Libraries were prepared using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB)
polyA selection, RNA-seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
reads were trimmed by removal of the adaptor sequences and to a length of 6-50 bases
reads mapping to the Drosophila melanogaster rRNA precursor and the mitochondrial genome using Bowtie (release 1.2.2) with 0 mismatches were excluded
reads were mapped to the Drosophila melanogaster genome using STAR (v.2.5.2b)
reads mapping to rRNA, tRNA, mitoRNA were excluded
Assembly: dm6
Supplementary files format and content: all mapping and unique mapping bigwig files for sense and antisense
|
| |
|
| Submission date |
May 08, 2022 |
| Last update date |
May 17, 2022 |
| Contact name |
Lisa Baumgartner |
| E-mail(s) |
lisa.baumgartner@imba.oeaw.ac.at
|
| Organization name |
IMBA
|
| Lab |
Brennecke Lab
|
| Street address |
Dr. Bohr-Gasse 3
|
| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
| |
|
| Platform ID |
GPL25244 |
| Series (2) |
| GSE202466 |
The Drosophila ZAD zinc finger protein Kipferl guides Rhino to piRNA clusters (RNA-Seq) |
| GSE202468 |
The Drosophila ZAD zinc finger protein Kipferl guides Rhino to piRNA clusters |
|
| Relations |
| BioSample |
SAMN28156084 |
| SRA |
SRX15206379 |
