|
Status |
Public on Mar 02, 2012 |
Title |
G9a f/f 12h pIC Rep1 |
Sample type |
RNA |
|
|
Source name |
MEF
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl/6 genotype: G9a f/f, ERT2-Cre passages: Passaged 12-15 times treatment: transfected with polyIC for 12h
|
Treatment protocol |
Primary MEFs were immortalized at passage 2. At passage 5, MEFs were treated witheither EtOH as a vehicle control or Tamoxifen to induce deletion of G9a. MEFs were transfected with polyIC using Lipofectamine 2000.
|
Growth protocol |
MEFs were grown in 10 cm plates with DMEM medium containing 15% FBS, Pen/Strep, Glutamine, NEAA and 2-mercaptoethanol. After immortalization with SV40-LTA, MEFs were grown in 15 cm plates with the same medium except FBS was 10%.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp Premier RNA Amplification kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
G9a f/f MEF transfected with polyIC for 12h
|
Data processing |
The data were normalised using percentile normalisation with GeneSpringGX 10
|
|
|
Submission date |
Oct 18, 2010 |
Last update date |
Mar 02, 2012 |
Contact name |
Terry Fang |
E-mail(s) |
tfang@rockefeller.edu
|
Phone |
212-327-8265
|
Fax |
212-327-8258
|
Organization name |
Rockefeller University
|
Street address |
1230 York Ave
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (2) |
GSE24776 |
Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response (WT and G9a deficient MEFs) |
GSE24826 |
Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response |
|