|
| Status |
Public on May 06, 2013 |
| Title |
Gene expression data from PBC of 47 years old male hypertensive |
| Sample type |
RNA |
| |
|
| Source name |
Human PBC (HTN)
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Peripheral blood
gender: male
age: 47 years
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using trizol reagent (invitrogen, USA) according to manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared according to the satandard Affymetrix from 10microgram of total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10microgram of cRNA were hybridized for 16 hrs at 45 degree celcius on GeneChip Human Genome Plus 2.0 Array. Gene
Gene chips were processed using Affymetrix's fludics station 400
|
| Scan protocol |
Gene chips were scanned using the Affymetrix scanner 300
|
| Description |
Gene expression data from PBC of 47 years old male hypertensive
|
| Data processing |
Images were processed and raw data were extracted with GeneChip operating software (GCOS 1.4). Data normalization was carried out with dChip.
|
| |
|
| Submission date |
Oct 18, 2010 |
| Last update date |
May 06, 2013 |
| Contact name |
MELVIN KORKOR |
| E-mail(s) |
melkorkor@yahoo.com
|
| Phone |
0086-13756681355
|
| Organization name |
JILIN UNIVERSITY
|
| Department |
INTERNAL MEDICINE
|
| Lab |
Bioassay Laboratory of capitalBio Corporation
|
| Street address |
18 Life Science Parkway, Changping District, Beijing,China
|
| City |
Beijing,China |
| ZIP/Postal code |
102206 |
| Country |
China |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE24752 |
Microarray Analysis of differential gene expression in peripheral blood cells of patients with human essential hypertension |
|
