|
| Status |
Public on Oct 08, 2010 |
| Title |
BT474 |
| Sample type |
RNA |
| |
|
| Source name |
Cultured cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: breast cancer cell
cell line: BT474
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Subconfuent breast cancer cell lines
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using Ambion MessageAmp Premier labeling kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A plus 2.0 arrays. Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 7G.
|
| Description |
Gene expression data from breast cancer cell lines
|
| Data processing |
The data were analyzed with RMA (Robust Multichip Average) using Bioconductor
|
| |
|
| Submission date |
Oct 07, 2010 |
| Last update date |
Oct 07, 2010 |
| Contact name |
Igor Shats |
| E-mail(s) |
is37@duke.edu
|
| Organization name |
Duke University
|
| Street address |
101 Science Drive
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (2) |
| GSE24578 |
Basal gene expression of breast cancer cell lines |
| GSE24717 |
Using a Stem Cell-Based Signature to Guide Therapeutic Selection in Cancer |
|
