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Sample GSM605919 Query DataSets for GSM605919
Status Public on Oct 08, 2010
Title BT474
Sample type RNA
Source name Cultured cell line
Organism Homo sapiens
Characteristics cell type: breast cancer cell
cell line: BT474
Treatment protocol No treatment
Growth protocol Subconfuent breast cancer cell lines
Extracted molecule total RNA
Extraction protocol RNeasy mini kit (Qiagen)
Label biotin
Label protocol Biotinylated cRNA were prepared using Ambion MessageAmp Premier labeling kit.
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A plus 2.0 arrays. Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Scanner 3000 7G.
Description Gene expression data from breast cancer cell lines
Data processing The data were analyzed with RMA (Robust Multichip Average) using Bioconductor
Submission date Oct 07, 2010
Last update date Oct 07, 2010
Contact name Igor Shats
Organization name Duke University
Street address 101 Science Drive
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
Platform ID GPL571
Series (2)
GSE24578 Basal gene expression of breast cancer cell lines
GSE24717 Using a Stem Cell-Based Signature to Guide Therapeutic Selection in Cancer

Data table header descriptions

Data table
1007_s_at 11.16801594
1053_at 9.254924701
117_at 5.551099762
121_at 8.025274577
1255_g_at 3.1558474
1294_at 5.737402401
1316_at 6.481983999
1320_at 4.900184697
1405_i_at 5.025523869
1431_at 4.757698236
1438_at 8.655824946
1487_at 9.177156054
1494_f_at 6.437300476
1598_g_at 8.531106529
160020_at 6.898068447
1729_at 8.981709934
177_at 5.01099856
1773_at 7.059222832
179_at 8.875830888
1861_at 8.853606915

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.

Supplementary file Size Download File type/resource
GSM605919.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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