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Sample GSM605917 Query DataSets for GSM605917
Status Public on Oct 08, 2010
Title MDA-MB-157
Sample type RNA
Source name Cultured cell line
Organism Homo sapiens
Characteristics cell type: breast cancer cell
cell line: MDA-MB-157
Treatment protocol No treatment
Growth protocol Subconfuent breast cancer cell lines
Extracted molecule total RNA
Extraction protocol RNeasy mini kit (Qiagen)
Label biotin
Label protocol Biotinylated cRNA were prepared using Ambion MessageAmp Premier labeling kit.
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A plus 2.0 arrays. Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Scanner 3000 7G.
Description Gene expression data from breast cancer cell lines
Data processing The data were analyzed with RMA (Robust Multichip Average) using Bioconductor
Submission date Oct 07, 2010
Last update date Oct 07, 2010
Contact name Igor Shats
Organization name Duke University
Street address 101 Science Drive
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
Platform ID GPL571
Series (2)
GSE24578 Basal gene expression of breast cancer cell lines
GSE24717 Using a Stem Cell-Based Signature to Guide Therapeutic Selection in Cancer

Data table header descriptions

Data table
1007_s_at 9.596208407
1053_at 9.042731844
117_at 5.838077118
121_at 8.347402644
1255_g_at 3.304847689
1294_at 6.326222828
1316_at 6.474832999
1320_at 5.326026783
1405_i_at 6.116928238
1431_at 4.542849608
1438_at 6.428800823
1487_at 8.420721157
1494_f_at 6.125750406
1598_g_at 10.66413003
160020_at 7.450205878
1729_at 8.644759706
177_at 5.553115086
1773_at 6.351303232
179_at 8.748522761
1861_at 8.496140991

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.

Supplementary file Size Download File type/resource
GSM605917.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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