|
| Status |
Public on Nov 10, 2010 |
| Title |
hFib2 fibroblasts, replicate 2 - gene expression |
| Sample type |
RNA |
| |
|
| Source name |
hFib2 fibroblasts, replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: hFib2 fibroblasts
|
| Treatment protocol |
differentiated cells were removed from ESC and iPSC cultures prior to RNA isolation
|
| Growth protocol |
ESCs and iPSCs were grown on MEFs in standard human ESC media (DMEM/F12, 20% KOSR, 2mM L-Glutamine, 0.1mM NEAA, 0.1mM b-mercaptoethanol, 10ng/ml bFGF), fibroblasts were grown in aMEM/10%FCS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocols for Human U133 Plus 2.0 Array
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocols for Human U133 Plus 2.0 Array
|
| Scan protocol |
Standard Affymetrix protocols for Human U133 Plus 2.0 Array
|
| Description |
Gene expression profiles of human fibroblasts
|
| Data processing |
RMA
|
| |
|
| Submission date |
Sep 03, 2010 |
| Last update date |
Nov 10, 2010 |
| Contact name |
Nataly Moran Cabili |
| E-mail(s) |
nmcabili@fas.harvard.edu
|
| Organization name |
Broad Institute ; Harvard University
|
| Street address |
7 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE23968 |
Large intergenic non-coding RNAs as novel modulators of reprogramming: ESCs, fibroblast, and fibroblast-derived iPSC (gene expression) |
| GSE24182 |
Large intergenic non-coding RNAs as novel modulators of reprogramming |
|
