|
Status |
Public on Nov 10, 2010 |
Title |
MSC fibroblasts - gene expression |
Sample type |
RNA |
|
|
Source name |
MSC fibroblasts
|
Organism |
Homo sapiens |
Characteristics |
cell type: MSC fibroblasts
|
Treatment protocol |
differentiated cells were removed from ESC and iPSC cultures prior to RNA isolation
|
Growth protocol |
ESCs and iPSCs were grown on MEFs in standard human ESC media (DMEM/F12, 20% KOSR, 2mM L-Glutamine, 0.1mM NEAA, 0.1mM b-mercaptoethanol, 10ng/ml bFGF), fibroblasts were grown in aMEM/10%FCS
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol
|
Label |
biotin
|
Label protocol |
Standard Affymetrix protocols for Human U133 Plus 2.0 Array
|
|
|
Hybridization protocol |
Standard Affymetrix protocols for Human U133 Plus 2.0 Array
|
Scan protocol |
Standard Affymetrix protocols for Human U133 Plus 2.0 Array
|
Description |
Gene expression profiles of human fibroblasts
|
Data processing |
RMA
|
|
|
Submission date |
Sep 03, 2010 |
Last update date |
Nov 10, 2010 |
Contact name |
Nataly Moran Cabili |
E-mail(s) |
nmcabili@fas.harvard.edu
|
Organization name |
Broad Institute ; Harvard University
|
Street address |
7 Cambridge Center
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02142 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE23968 |
Large intergenic non-coding RNAs as novel modulators of reprogramming: ESCs, fibroblast, and fibroblast-derived iPSC (gene expression) |
GSE24182 |
Large intergenic non-coding RNAs as novel modulators of reprogramming |
|