|
| Status |
Public on Sep 29, 2022 |
| Title |
cell transfected siJunB-848, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
exponentially growing U2OS cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U2OS-derived UTA6 cells
cell type: osteosarcoma cell
treatment: siJunB-848
genotype: JUNB depletion
|
| Treatment protocol |
cells were transfected with siRNAs at a final concentration of 8 nM using Interferine (Polyplus) according to manufacturer’s instructions
|
| Growth protocol |
U2OS-derived UTA6 cells were seeded at subconfluence in DMEM medium supplemented with 10% foetal bovine serum and penicillin/streptomycin (100µg/ml each) in a humidified 5% CO2 atmosphere at 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted 48 h post-transfection using High Pure RNA Isolation kit (Roche). Triplicate RNA samples for each conditions were quality checked (RIN>9) with Eukaryotic Total RNA Nano Kit on Bioanalyser 2100 (Agilent) and then analysed on GeneChip Human Transcriptome Array 2.0 (Affymetrix)
|
| Label |
biotin
|
| Label protocol |
Label protocol were prepared according to the standard Affymetrix protocol. See manufacturer's web site
|
| |
|
| Hybridization protocol |
Hybridization was performed in the Affymetrix Human Gene 2.0 ST Array.
|
| Scan protocol |
Scan was performed according to Affymetrix instructions (user manual P/N 702731 Rev. 3).
|
| Description |
Gene expression data from JUNB depleted exponentially growing U2OS cells
JB3_s10
|
| Data processing |
Data were processed with Partek Suite and differentially expressed genes detected with the LIMMA package.
Significantly differentially expressed genes were selected based on a false discovery rate (FDR) threshold of less than 0.05.
Log2 probe set signal intensities after RMA normalization were generated.
|
| |
|
| Submission date |
Feb 02, 2022 |
| Last update date |
Sep 29, 2022 |
| Contact name |
Rosa Farràs |
| E-mail(s) |
rfarras@cipf.es
|
| Phone |
+34963289680
|
| Organization name |
Centro de Investigación Príncipe Felipe
|
| Street address |
Eduardo Primo Yúfera, 12
|
| City |
Valencia |
| State/province |
Valencia |
| ZIP/Postal code |
46012 |
| Country |
Spain |
| |
|
| Platform ID |
GPL16686 |
| Series (2) |
| GSE195987 |
Gene expression data from JUNB depleted exponentially growing U2OS-derived UTA6 cells |
| GSE195993 |
JUNB transcriptional regulation in cell cycle control and tumorigenic cell invasion |
|
