|
| Status |
Public on Sep 29, 2024 |
| Title |
yw_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
First instar laeva
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell type: Whole body
genotype: yw
extract_protocol: RNA extraction
|
| Treatment protocol |
Fly culture and crosses were carried out at room temperature.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
First instar larvae were collected and extract total RNA by RNA basic kit (FastGene) according to the manufacturer protocol.
Total RNA libraries were prepared by using SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Clontech).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Basecalling with Sanger / Illumina 1.9
RNA sequence reads were analyzed on the Galaxy web platform (usegalaxy.eu). After trimming of the adaptor sequence, quality was controlled by FastQC 0.11.2.
Reads were mapped to Drosophila melanogaster BDGP6.28 genome using STAR (Version 2.7.0a)
Mapped reads were counted using featureCounts
Genome_build: Drosophila melanogaster BDGP6.28
Supplementary_files_format_and_content: text files contain three columns: 1. transcript name;2.read counts
|
| |
|
| Submission date |
Dec 30, 2021 |
| Last update date |
Sep 29, 2024 |
| Contact name |
Eriko Matsuura |
| E-mail(s) |
eriko.daichi.55@gmail.com
|
| Organization name |
The University of Tokyo
|
| Department |
IQB
|
| Street address |
Yayoi
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-0032 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17275 |
| Series (1) |
| GSE192811 |
miRNA-mediated gene silencing in Drosophila larval development involves GW182-dependent and independent mechanisms |
|
| Relations |
| BioSample |
SAMN24526244 |
| SRA |
SRX13558045 |
