|
| Status |
Public on May 01, 2011 |
| Title |
SS gland, early, patient 20, batch 2 |
| Sample type |
RNA |
| |
|
| Source name |
minor salivary gland
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: minor salivary gland
disease status: early SS
|
| Treatment protocol |
Minor salivary glands were removed for routine diagnostic procedures
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction using Trizol Reagent and further purification using Qiagen Rneasy mini-kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
|
| Description |
RNA from minor salivary gland
|
| Data processing |
Affymetrix Microarray Suite version 5.0 using Affymetrix software EC (Experssion Console 1.1)
|
| |
|
| Submission date |
Jul 23, 2010 |
| Last update date |
Jun 22, 2021 |
| Contact name |
Niki Moutsopoulos |
| E-mail(s) |
nmoutsopoulos@dir.nidr.nih.gov
|
| Organization name |
NIDCR
|
| Street address |
30 convent Dr
|
| City |
Bethesda |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE23117 |
Gene expression in minor salivary gland of patients with Sjogren's syndrome (SS) and control |
|
