|
| Status |
Public on Nov 30, 2013 |
| Title |
HNORPC30 |
| Sample type |
RNA |
| |
|
| Source name |
colon biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
tissue: colon biopsy
disease status: patient carrier of mutations predisposed to HNPCC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified from either surgical samples or five pooled pinch biopsies taken from the right colon using the RNA Purification Reagent (Promega, Madison, WI) as suggested by the manufacturer. RNAs were then quantified and inspected by Bioanalyzer (Agilent Technologies, Waldbrom, Germany) analysis
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
patient carrier of mutations predisposed to HNPCC
|
| Data processing |
The data were normalized using the locally weighted scatterplot smoothing (LOWESS) algorithm. Analysis was performed using oneChannelGUI a package belonging to Bioconductor release 2.6.
|
| |
|
| Submission date |
Jul 19, 2010 |
| Last update date |
Nov 30, 2013 |
| Contact name |
Raffaele A Calogero |
| E-mail(s) |
raffaele.calogero@unito.it
|
| Phone |
++39 0116706454
|
| Organization name |
University of Torino
|
| Department |
Molecular Biotechnology Center
|
| Lab |
Bioinformatics and Genomics Unit
|
| Street address |
Via Nizza 52
|
| City |
Torino |
| State/province |
To |
| ZIP/Postal code |
10126 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE23011 |
Transcription profile of colon biopsies from normal (NOR) donors, patients, underwent resection for CRC (M-CRC) and patients carriers of mutations predisposed to HNPCC (HNPC) |
|
