|
| Status |
Public on Jul 01, 2011 |
| Title |
HBL-1 shIRF4_3'UTR 48h - mAdbID:99873 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
HBL-1 shControl - 48 hr
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
cell line: HBL-1
disease state: B cell-like (ABC) subtype of diffuse large B cell lymphoma (DLBCL)
|
| Treatment protocol |
Treatment type: shRNA transduction
Treatment time: 48 hours
In-vitro treatment: Cells were transduced with retroviral vectors expressing shControl. shRNA expression was induced with doxycycline for 48 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol Extraction Protocol
Other: Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA).
|
| Label |
cy3
|
| Label protocol |
Cy3 Labeling Protocol
Other: A 1 ug of total RNA was labeled with Cy3.
|
| |
|
| Channel 2 |
| Source name |
HBL-1 shIRF4_3'UTR - 48 hr
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
cell line: HBL-1
disease state: B cell-like (ABC) subtype of diffuse large B cell lymphoma (DLBCL)
|
| Treatment protocol |
Treatment type: shRNA transduction
Treatment time: 48 hours
In-vitro treatment: Cells were transduced with retroviral vectors expressing shIRF4_3'UTR. shRNA expression was induced with doxycycline for 48 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol Extraction Protocol
Other: Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA).
|
| Label |
cy5
|
| Label protocol |
Cy5 Labeling Protocol
Other: A 1 ug of total RNA was labeled with Cy5.
|
| |
|
| |
| Hybridization protocol |
Agilent Hybridization Protocol
Other: According to the manufacture's recommended protocol (Two-Color Microarray-Based Gene Expression Analysis, Agilent).
|
| Scan protocol |
Agilent Scanning Protocol
Other: Arrays were scanned at 5um resolution on an Agilent DNA Microarray Scanner (G2505C, Agilent) using the default settings for 4x44k format two-color arrays.
|
| Description |
mAdb experiment ID: 99873
|
| Data processing |
Agilent Data Processing Protocol
Calculation Method: Images were auto gridded, analyzed and data extracted using Agilent Feature Extraction Software (Version 10.1.1.1). Spot values were normalized using the default linear-lowess normalization.
|
| |
|
| Submission date |
Jul 12, 2010 |
| Last update date |
Sep 28, 2011 |
| Contact name |
Louis M. Staudt |
| E-mail(s) |
lstaudt@mail.nih.gov
|
| Phone |
301-402-1892
|
| Organization name |
National Cancer Institute
|
| Department |
Lymphoid Malignancies Branch
|
| Lab |
Louis M Staudt
|
| Street address |
9000 Rockville Pike, Bldg 10, Rm 4N114
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (2) |
| GSE22901 |
Determining IRF4 target genes using RNA interference in ABC-DLBCL and MM |
| GSE32456 |
Co-addiction to IRF4 and SPIB in ABC-DLBCL |
|
