|
| Status |
Public on May 16, 2023 |
| Title |
HYB025 |
| Sample type |
RNA |
| |
|
| Source name |
blood
|
| Organism |
Homo sapiens |
| Characteristics |
treatment arm: Placebo
treatment dose: Placebo
tissue: whole blood
timepoint: 96 weeks
|
| Treatment protocol |
Blood samples for DNA extraction were collected in EDTA tubes.
|
| Growth protocol |
Not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs are extracted from whole blood using the PAXgene RNA Blood RNA kit from Qiagen
|
| Label |
biotin
|
| Label protocol |
Biotin
|
| |
|
| Hybridization protocol |
Following fragmentation of DNA, labeled DNA was hybridized over night on Affymetrix Human Genome U133 Plus 2.0 microarray, the hybridized array was stained and washed using an automated protocol on the fluidics station.
|
| Scan protocol |
After hybridization and staining, the Affymetrix arrays were scanned to create image data (DAT) files. The GeneChip Command Console Software then automatically aligned a grid on the DAT files and computed the Cell Intensity data (CEL) file.
|
| Data processing |
Probe level analysis was performed on the Affymetrix raw data (.cel files) with the robust multichip average (RMA) method implemented in the Bioconductor package to obtain a log2 expression value for each gene probe set.
|
| |
|
| Submission date |
Oct 12, 2021 |
| Last update date |
May 16, 2023 |
| Contact name |
Irina Kalatskaya |
| E-mail(s) |
irina.kalatskaya@emdserono.com
|
| Organization name |
EMD Serono Research and Development Institute
|
| Department |
Clinical Measurement Sciences
|
| Street address |
45 Middlesex Turnpike
|
| City |
Billerica |
| State/province |
MA |
| ZIP/Postal code |
01821 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE185773 |
CLARITY study: Expression data from RRMS patients treated with Oral Cladribine versus Placebo at week 96 |
|
