|
| Status |
Public on May 13, 2011 |
| Title |
PEO4 Estrogen Tx rep3 |
| Sample type |
RNA |
| |
|
| Source name |
PEO4 (ER+) ovarian cancer cells tagged with ZsGreen and laser captured from intraperitoneal xenografts of a mouse treated with estrogen
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: PEO4 (ER+) ovarian cancer cells
protocol: human ovarian cancer cells xenografted into female ovariectomized athymic nude mice
agent: Estrogen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from the laser captured human cells using the Picopure RNA Isolation kit (Molecular Devices)
|
| Label |
biotin
|
| Label protocol |
RNA was amplified with WT-Ovation FFPE RNA Amplification System V2 (NuGEN, San Carlos, CA ) and biotin labeled using the FL-Ovation cDNA Biotin Module V2 (NuGEN)
|
| |
|
| Hybridization protocol |
Samples were hybridized to U133 2+ whole human genome expression arrays in the University of Colorado Comprehensive Cancer Center Microarray Core according to their standard protocol
|
| Scan protocol |
Samples were scanned in the University of Colorado Comprehensive Cancer Center Microarray Core according to their standard protocol
|
| Description |
n/a
|
| Data processing |
Samples were assayed for quality and to check for MAS 5.0 present calls on at least one chip using R and then RMA normalized and analyzed using Partek
|
| |
|
| Submission date |
Jun 28, 2010 |
| Last update date |
May 13, 2011 |
| Contact name |
Monique Spillman |
| E-mail(s) |
Monique.Spillman@ucdenver.edu
|
| Organization name |
University of Colorado Denver
|
| Street address |
Mail Stop B198-4, PO Box 6511
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE22600 |
Tissue Specific Pathways for Estrogen Regulation of Ovarian Cancer Growth and Metastasis |
|
