|
| Status |
Public on Jun 30, 2010 |
| Title |
DBDmut-24 Hr E2_111A |
| Sample type |
RNA |
| |
|
| Source name |
MDA-MB-231 cells
|
| Organism |
Homo sapiens |
| Characteristics |
genotype/variation: DBDmut-ER
time point: 24 Hr E2
|
| Treatment protocol |
Cells were treated with 10 nM E2 for 1-24 hours.
|
| Growth protocol |
Cells were grown in phenol-red free DMEM-Ham's F-12 media with 10 mM HEPES, 10% Charcoal-dextran stripped media, 6 ng/ml bovine insulin, 3.75 ng/ml Hydrocortisone and 16 ug/ml glutathione.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Breast Cancer Cells Stably expressing a Mutant Estrogen Receptor
|
| Data processing |
log transformed gc-RMA data from the CEL files processed using the affy and gcrma package protocols in R/BioConductor
|
| |
|
| Submission date |
Jun 28, 2010 |
| Last update date |
Jun 29, 2010 |
| Contact name |
Joshua D Stender |
| Organization name |
University of California at San Diego
|
| Street address |
9500 Gillman
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (2) |
| GSE22593 |
WT and DBDmut Breast Cancer Cells |
| GSE22610 |
Genome-Wide Analysis of Estrogen Receptor-α DNA Binding and Tethering Mechanisms |
|
