|
| Status |
Public on Jul 09, 2010 |
| Title |
1174 DN2L condition cell line |
| Sample type |
genomic |
| |
|
| Source name |
1174 DN2L
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: 1174 DN2L
tissue: brain
|
| Growth protocol |
Cell lines were grown in DN2L conditions (DMEM-F12 supplemented with 20ng/ml epidermal growth (EGF; Invitrogen), 20ng/ml basic fibroblast growth factor (bFGF; Invitrogen), 1:50 B27 supplement (Invitrogen), 1:100 N2 supplement (Invitrogen), 10ng/ml leukemia inhibitory factor (LIF; Chemicon)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from primary tissue and cell lines using the Qiagen DNA Mini Kit after disruption with a Qiagen Tissue Lyser. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Description |
SNP and CNV data
|
| Data processing |
CEL files and were processed by Partek Genomics Suite.
Genetic CN events from Affymetrix chips were reported based on CN values using Partek’s Genomic Segmentation algorithm. Single nucleotide polymorphism (SNP) genotype data was generated through Affymetrix’s Genotyping Console then imported into Partek and compared against a 270 HapMap genotype sample dataset
|
| |
|
| Submission date |
Jun 25, 2010 |
| Last update date |
Jul 08, 2010 |
| Contact name |
Huidong Shi |
| E-mail(s) |
hshi@augusta.edu
|
| Phone |
706-721-6000
|
| Organization name |
Augusta University
|
| Department |
Georgia Cancer Center
|
| Lab |
2125 K
|
| Street address |
1120 15th Street, CN2138
|
| City |
Augusta |
| State/province |
GA |
| ZIP/Postal code |
30912 |
| Country |
USA |
| |
|
| Platform ID |
GPL6804 |
| Series (1) |
| GSE22577 |
Meningioma initiating cells (MICs) derived from primary tumors |
|
