|
| Status |
Public on Jun 23, 2010 |
| Title |
BDC Treg #2 |
| Sample type |
RNA |
| |
|
| Source name |
T cells were sorted from the spleen of BDC2.5 RAG+/o NOD without treatment
|
| Organism |
Mus musculus |
| Characteristics |
strain: NOD
genotype/variation: BDC2.5 TCR tg RAG1+/o Foxp3GFP NOD
age: 28 days old
number of mice per chip: one
gender: female
tissue: spleen
cell type: B220-, CD8a-, CD11b/c-, CD4+,GFP+ T cells
protocol: no treatment
|
| Treatment protocol |
Anti-CD3 treatment (37.5 ug of anti-CD3 mAb (clone:KT3) was iv injected for 5 consecutive days from 17 days of age) or no treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
|
| Label |
biotin
|
| Label protocol |
Biotin
|
| |
|
| Hybridization protocol |
Biotin-labeled cRNA was fragmented and hybridized to the array in a cocktail containing control oligos at 45°C for 16 hours, rotated at 60rpm, in the Affymetrix GeneChip Hybridization Oven 640. After hybridization, the arrays were automatically cycled through buffer washes and stained with streptavidin R-phycoerythrin conjugate (Invitrogen) in the Affymetrix GeneChip Fluidics Station 400 (EukGE-WS2v4 protocol).
|
| Scan protocol |
After staining and washing, the arrays were scanned in the Affymetrix GeneChip Scanner 3000 using the Affymetrix GCOS (GeneChip Operating Software) to automatically generate .CEL files.
|
| Description |
jos4621.CEL
|
| Data processing |
Raw data were preprocessed with the RMA algorithm in GenePattern, and averaged expression values were used for analysis.
|
| |
|
| Submission date |
Jun 23, 2010 |
| Last update date |
Jun 23, 2010 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE22527 |
Anti-CD3 therapy permits regulatory T cells to surmount T cell receptor-specified peripheral niche constraints |
|
