|
Status |
Public on Sep 21, 2021 |
Title |
Western diet-fed SIRT1 LKO x AAV8-NAMPT4 |
Sample type |
SRA |
|
|
Source name |
Liver
|
Organism |
Mus musculus |
Characteristics |
strain: C57B/6J tissue: Liver age: 20wk
|
Treatment protocol |
Mice were injected with AAV8 encoding empty vector or SIRT1
|
Growth protocol |
Littermate WT and SIRT LKO mice were grown to adulthood ~8wks of age
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol reagent was used to isolate total RNA, performed exactly per manufacturer protocol on 50-100mg liver tissue homogenate oligo-dT
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Basecalling with Illumina RTA 1.9 Demultiplexing with Illumina bcl2fastq2 Fastq files were combined and aligned with STAR 2.5.1a Gene counts quantitated with Subread:featureCount 1.4.6-p5 with CPM and RPKM transformations added with custom Rscripting. Genome_build: Ensembl GRCm38.76 Supplementary_files_format_and_content: Gene counts with CPM and RPKM in tab-delimitted text format
|
|
|
Submission date |
Sep 18, 2021 |
Last update date |
Sep 21, 2021 |
Contact name |
Brian DeBosch |
E-mail(s) |
deboschb@wustl.edu
|
Phone |
314-747-2692
|
Organization name |
Washington University School of Medicine
|
Department |
Pediatrics and Cell Biology & Physiology
|
Lab |
Brian DeBosch
|
Street address |
660 South Euclid Avenue
|
City |
St. Louis |
State/province |
Missouri |
ZIP/Postal code |
63110 |
Country |
USA |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE184394 |
Role of SIRT1 in NAMPT-induced liver gene expression |
|
Relations |
BioSample |
SAMN21502437 |
SRA |
SRX12252646 |