|
| Status |
Public on Jun 20, 2012 |
| Title |
DGM-00583 [genotype] |
| Sample type |
genomic |
| |
|
| Source name |
Blood from healthy nonsmokers or healthy smokers
|
| Organism |
Homo sapiens |
| Characteristics |
department of genetic medicine id: DGM-00583
smoking status: S
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from blood samples using the Autogen FX robotic system in according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
DNA labeling was done according to the standard Affymetrix protocol from 50 nanograms total DNA (Affymetrix® Genome-Wide Human SNP Nsp/Sty Assay 5.0, 702419 Rev.1, Affymetrix).
|
| |
|
| Hybridization protocol |
Following labelling, 50 nanograms of DNA were hybridized for 16 hr at 50C on Affymetrix GeneChip Genome-Wide Human SNP Array 5.0 . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
n/a
|
| Data processing |
The data were analyzed with Partek Genomics Suite software, version 6.2 using default analysis settings and global scaling as normalization method.
CHP files were created from CEL files after running BRLMM_p
|
| |
|
| Submission date |
Jun 03, 2010 |
| Last update date |
Jun 20, 2012 |
| Contact name |
Yael Strulovici-Barel |
| E-mail(s) |
yas2003@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Department of Genetic Medicine
|
| Lab |
Crystal
|
| Street address |
1300 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL6804 |
| Series (1) |
| GSE22047 |
Modulation of Cystatin A Expression in Human Airway Epithelium Related to Genotype, Smoking COPD and Lung Cancer |
|
