Total RNA was isolated using the miRVana microRNA isolation kit (Ambion).
Label
FAM
Label protocol
standard Taqman assay
Hybridization protocol
not applicable
Scan protocol
not applicable
Description
The embryonic human pancreas were embeded in 10% volume of RNAlater solution (Ambion).
Data processing
Data was processed using the softwares SDS 2.3 and RQ manager 1.2 from Applied Biosystems. dCt for delta Ct, defined by the formula (Ct of the specific miRNA-Ct of the endogenous control RNU48) for each sample. RQ for relative quantification, defined by the formula 2^-dCt . Small nuclear RNA (RNU48) was used as endogenous control.
