|
| Status |
Public on Nov 01, 2010 |
| Title |
HepG2 Negative Control siRNA 2 |
| Sample type |
RNA |
| |
|
| Source name |
HepG2 Negative Control siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Liver cancer cell line
cell line: HepG2
sirna: Negative Control siRNA
|
| Treatment protocol |
15 nM siRNA for 48 h
|
| Growth protocol |
DMEM/F-12 (+10% fetal bovine serum)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol reagent. Quality control and measurement were performed using NanoDrop and Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Totalprep RNA Amplification kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
Data was processed by using Gene Expression Module v 1.1.1 in Genome Studio V2009.1 from illumina, Inc. The background noise was substrated and quantile normalization was applied.
|
| |
|
| Submission date |
May 21, 2010 |
| Last update date |
Oct 04, 2010 |
| Contact name |
Daekwan Seo |
| E-mail(s) |
daekwan_seo@nih.gov
|
| Phone |
301-496-5688
|
| Fax |
301-496-0734
|
| Organization name |
NIH
|
| Department |
NCI
|
| Lab |
LEC
|
| Street address |
37 Convent Dr. Rm 4140
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20878 |
| Country |
USA |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE21955 |
COP1 siRNA knockdown in human Hepatocellular carcinoma cells |
|
