|
| Status |
Public on Apr 20, 2011 |
| Title |
colmut_1 with array type ATH1 from Affymetrix |
| Sample type |
RNA |
| |
|
| Source name |
colmut_1
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: columbia
developmental stage (boyes et al. plant cell 2001): 1-5 rosette leaf
|
| Treatment protocol |
Name:ColMut - environmental treatment - pathogen infection,inoculation with bacteria pto expressing the mutant effector hopz1ac216a.:quantity 10cfu/ml time 6hour . - Leaves were infiltrated with bacterial suspension containing 108 cfu/ml of Pseudomonas syringae pv. tomato (Pto) expressing the effector HopZ1a carrying a mutation that inactivates its catalytic site (C216A), using a blunt syringae
|
| Growth protocol |
caulin leaf - -Grown in rich substrate in growth chambers at 21 ºC with 8h light / 16h dark.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
colmut_1:7.5ng. Qiagen_RNeasy kit
|
| Label |
Biotin
|
| Label protocol |
labelling Biotin direct, amplification=yes, cRNA 20 ug.
|
| |
|
| Hybridization protocol |
labelled extract quantity: 15ug
|
| Scan protocol |
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
|
| Description |
- Pseudomonas syringae is a plant pathogenic bacterium that relies on a type III secretion system (T3SS) to cause disease in susceptible hosts and to trigger defence in resistant plants. The T3SS translocates effector proteins directly inside the plant cell. Some P. syringae pathovars translocate the effector HopZ1a, which is recognized in Arabidopsis, triggering the hypersensitive response (HR). This resistance does not depend on the usual defence pathways involved in resistance against other avirulence effectors, so it would be very helpful to know the transcriptomic events that take place in the context of a hypersensitive response triggered by HopZ1a. ULP genes codifies for plant SUMO-proteases. Recent studies by our group revealed that they could be involved in plant defense against microorganisms.
|
| Data processing |
The data were normalized with the gcrma algorithm (Irizarry et al., 2003) using R-2.6.2 version and AFFYMETRIX bioconductor packages (Gentleman and Carey, 2002).
|
| |
|
| Submission date |
May 20, 2010 |
| Last update date |
Aug 15, 2018 |
| Contact name |
stephanie huguet |
| E-mail(s) |
huguet@evry.inra.fr
|
| Organization name |
inra
|
| Street address |
2 rue gaston cremieux
|
| City |
evry |
| ZIP/Postal code |
91000 |
| Country |
France |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE21920 |
affy_syringae_malaga_athaliana-Role of different proteases in plant defence |
|
| Relations |
| Reanalyzed by |
GSE118579 |
