|
| Status |
Public on Jun 07, 2010 |
| Title |
HuES 3 |
| Sample type |
RNA |
| |
|
| Source name |
human ES
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human embryonic stem cells
|
| Treatment protocol |
no special treatment
|
| Growth protocol |
Human ES and iPS were grown in standard conditions with FGF on feeder MEFs. Human LR5-iPS were grown in the presence of human LIF and Doxycycline inducible expression of 5 reprogramming factors: OCT4, SOX2, KLF4, MYC and NANOG.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were size-sorted by forward scatter and side scatter on FACS-ARIA II. Total RNA of the sorted cells were extracted by TRIzol following the manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
1 microgram of the total RNA from each sample was labelled by the Agilent QuickAmp 1-Color Labeling Kit following the manufacturer's protocols.
|
| |
|
| Hybridization protocol |
Cy-3 labelled cRNA was hybridized using Agilent Hybridization Kit following the manufacturer's protocols.
|
| Scan protocol |
The washed microarray slides were scanned in Agilent Technologies Scanner following the manufacturer's protocols.
|
| Description |
US45102879_251485044614_S01_GE1-v5_95_Feb07_1_1.txt
|
| Data processing |
The background subtracted signal and the signal to background ratio of each feature were uploaded into GeneSifter gene expression analysis program. The projects were median-normalized before analysis. The data uploaded here are original files prior to median normalization. The GeneSifter processed data is linked as a supplementary file to GSE21792.
|
| |
|
| Submission date |
May 12, 2010 |
| Last update date |
May 12, 2010 |
| Contact name |
Niels Geijsen |
| Organization name |
Massachusetts General Hospital
|
| Department |
Center for Regenerative Medicine
|
| Street address |
185 Cambridge St. CPZN 4-4256
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE21792 |
A murine-ES like state facilitates transgenesis and homologous recombination in human pluripotent stem cells |
|
