|
Status |
Public on Jul 02, 2021 |
Title |
Seven days old, FruLexA/Fru440 mutant - replicate 2 |
Sample type |
SRA |
|
|
Source name |
Seven days old, FruLexA/Fru440 mutant
|
Organism |
Drosophila melanogaster |
Characteristics |
replicate: 2 age: 7 days Sex: male housing: group genotype: FruLexaFru440 tissue: antennae
|
Treatment protocol |
For single housing (SH) condition, 80-100 hour-old pupae were separated by sex and males were placed into individual vials, allowed to eclose alone, and aged to 7 days to deprive flies of pheromone interaction on ORNs. For group housing (GH) condition, 25-30 newly eclosed males were collected and placed into food vials. These were aged to 7 days and 180 antennae were dissected per sample.
|
Growth protocol |
Flies were raised on standard fly food (containing yeast, cornmeal, agar, and molasses) at 25°C in a 12-hour light/12-hour dark cycle in cylindrical plastic vials (diameter, 24 mm and height, 94 mm).
|
Extracted molecule |
total RNA |
Extraction protocol |
KAPA HYPER mRNA , NON stranded. Illumina NovaSeq 6000.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
FRULEXAFRU440_2
|
Data processing |
Sequenced reads were filtered/trimmed with FASTP. Filtered reads were aligned to the dm6 reference genome (consolidated chromosomes only) using Mapsplice2 with default parameters. Samtools was used to filter alignments for read duplication ( rmdup -rS) and quality ( view -bh -q 20 -F 0x0200 -F 0x04 -f 0x0002 ). Mapped reads were counted against the reference gene annotation using featureCounts ( -J —verbose -M -p -B -C ). Genome_build: dm6 Supplementary_files_format_and_content: new_sequence.vs_dm6main.dm6_genes.mapspliceMulti.MpBC.counts.txt: Matrix table with raw gene counts for every gene and every sample.
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|
|
Submission date |
Jun 30, 2021 |
Last update date |
Jul 02, 2021 |
Contact name |
Corbin Jones |
E-mail(s) |
cdjones@email.unc.edu
|
Organization name |
UNC-CH
|
Department |
Biology
|
Street address |
250 Bell Tower Dr
|
City |
Chapel Hil |
State/province |
NC |
ZIP/Postal code |
27514 |
Country |
USA |
|
|
Platform ID |
GPL25244 |
Series (1) |
GSE179213 |
Changes in splicing and neuromodulatory gene expression programs in sensory neurons with pheromone signaling and social experience |
|
Relations |
BioSample |
SAMN19971755 |
SRA |
SRX11317314 |