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Sample GSM5411559 Query DataSets for GSM5411559
Status Public on Jul 02, 2021
Title Seven days old, FruLexA/Fru440 mutant - replicate 2
Sample type SRA
 
Source name Seven days old, FruLexA/Fru440 mutant
Organism Drosophila melanogaster
Characteristics replicate: 2
age: 7 days
Sex: male
housing: group
genotype: FruLexaFru440
tissue: antennae
Treatment protocol For single housing (SH) condition, 80-100 hour-old pupae were separated by sex and males were placed into individual vials, allowed to eclose alone, and aged to 7 days to deprive flies of pheromone interaction on ORNs. For group housing (GH) condition, 25-30 newly eclosed males were collected and placed into food vials. These were aged to 7 days and 180 antennae were dissected per sample.
Growth protocol Flies were raised on standard fly food (containing yeast, cornmeal, agar, and molasses) at 25°C in a 12-hour light/12-hour dark cycle in cylindrical plastic vials (diameter, 24 mm and height, 94 mm).
Extracted molecule total RNA
Extraction protocol KAPA HYPER mRNA , NON stranded.
Illumina NovaSeq 6000.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description FRULEXAFRU440_2
Data processing Sequenced reads were filtered/trimmed with FASTP.
Filtered reads were aligned to the dm6 reference genome (consolidated chromosomes only) using Mapsplice2 with default parameters.
Samtools was used to filter alignments for read duplication ( rmdup -rS) and quality ( view -bh -q 20 -F 0x0200 -F 0x04 -f 0x0002 ).
Mapped reads were counted against the reference gene annotation using featureCounts ( -J —verbose -M -p -B -C ).
Genome_build: dm6
Supplementary_files_format_and_content: new_sequence.vs_dm6main.dm6_genes.mapspliceMulti.MpBC.counts.txt: Matrix table with raw gene counts for every gene and every sample.
 
Submission date Jun 30, 2021
Last update date Jul 02, 2021
Contact name Corbin Jones
E-mail(s) cdjones@email.unc.edu
Organization name UNC-CH
Department Biology
Street address 250 Bell Tower Dr
City Chapel Hil
State/province NC
ZIP/Postal code 27514
Country USA
 
Platform ID GPL25244
Series (1)
GSE179213 Changes in splicing and neuromodulatory gene expression programs in sensory neurons with pheromone signaling and social experience
Relations
BioSample SAMN19971755
SRA SRX11317314

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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