|
| Status |
Public on Mar 26, 2024 |
| Title |
10_FH-W_CCS: Familial hypercholesterolemia patients with coronary calcium score_r5 |
| Sample type |
RNA |
| |
|
| Source name |
Plasma
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Familial hypercholesterolemia
genotype: Patients carrying the heterozygous mutation in the LDLR gene
coronary calcium score condition: FH-W_CCS
gender: Male
age: 57
tissue: Plasma
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Familial hypercholesterolemia patients plasma (200uL) was used for isolation of circulating miRNAs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
To isolate plasma miRNAs, we used plasma from familial hypercholesterolemia patients and miRNeasy Serum/Plasma Advanced Kit (Qiagen Inc), according to manufacturer’s instructions.
|
| Label |
Biotin
|
| Label protocol |
We used Affymetrix FlashTag Biotin HSR RNA Labeling Kit (Affymetrix, Rev3) to label 8 ul of total RNA isolated with miRNeasy Serum/Plasma Advanced Kit (Qiagen Inc) from each sample, according to manufacturers instructions. RNA quantitation was not considered due to low levels of total RNA isolated from plasma.
|
| |
|
| Hybridization protocol |
Hybridization was performed on Affymetrix miRNA 4.0 arrays and cartridges were incubated at 48°C with 60 rpm rotation for 48 h using Affymetrix Hybridization Oven 600. After, each array was washed and stained using the FS450_002 fluidics script in Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned by using the GeneChip scanner 3000 7G System
|
| Description |
Circualting miRNA data in presence of coronary calcium score
|
| Data processing |
The data were explored using the Transcriptome Analysis Console software (TAC 4.0) for data summarization and normalization by RMA+DABG procedure (implemented in TAC 4.0 software).
|
| |
|
| Submission date |
Jun 14, 2021 |
| Last update date |
Apr 26, 2024 |
| Contact name |
Lina Badimon |
| E-mail(s) |
lbadimon@santpau.cat
|
| Organization name |
Research Institut- HSCSP
|
| Department |
ICCC-Cardiovascular Program
|
| Street address |
Carrer de Sant Antoni Maria Claret, 167
|
| City |
Barcelona |
| State/province |
BARCELONA |
| ZIP/Postal code |
08025 |
| Country |
Spain |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE178126 |
Circulating miRNA profile and coronary calcification in asymptomatic patients with familial hypercholesterolemia. |
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