|
| Status |
Public on Oct 23, 2010 |
| Title |
2-Muscle_Unloading_0h |
| Sample type |
RNA |
| |
|
| Source name |
vastus lateralis, control
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: vastus lateralis
protocol: control
time: 0 hour
|
| Treatment protocol |
Seven sedentary men (n = 7, mean age ± S.D = 22.1 ± 3.7 yr) following 48h unloading (UL) via unilateral lower limb suspension and 24h reloading (RL).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 plus2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
|
| |
|
| Submission date |
Apr 23, 2010 |
| Last update date |
Dec 28, 2010 |
| Contact name |
Yi-Wen Chen |
| E-mail(s) |
ychen@cnmc.org
|
| Organization name |
Children's Research Institute
|
| Department |
Genetic Medicine Research
|
| Street address |
111 Michigan Ave NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20010 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE21496 |
Effects of 48h Lower Limb Unloading in Human Skeletal Muscle |
|
