|
| Status |
Public on May 01, 2011 |
| Title |
SG200_drep1_CC-MMglc_w-AA_48h_3 |
| Sample type |
RNA |
| |
|
| Source name |
Deletion of gene rep1 (um03924) in SG200 background, nourseothricin replacement, grown on NM+ charcoal plates for 48 h.
|
| Organism |
Mycosarcoma maydis |
| Characteristics |
genotype: SG200∆rep1
genetic background: U. maydis strain SG200 (Bölker et al., 1995) is a haploid solopathogenic FB1 derivative
|
| Growth protocol |
For aerial hyphae formation cells were grown for 48h at 22 °C on nitrate minimal medium (NM+) (Holliday, 1974) containing 2 % agar (w/v), 1 % charcoal (w/v), 20 mg l-1 histidine, 380 mg l-1 leucine, 20 mg l-1 tryptophan, 50 mg l-1 uracil and 76 mg l-1 Yeast Synthetic Drop-out Medium Supplements (Sigma, Y2001). This was performed for three independently conducted replicates
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were frozen in liquid nitrogen for three independently conducted replicates.
Total RNA was extracted using TRIZOL (Invitrogen) according to the manufacturers instructions. RNA samples were column purified (Qiagen RNeasy) and the quality checked using a Bioanalyzer with a RNA 6000 Nano LabChip kit (Agilent Technologies).
|
| Label |
Biotin
|
| Label protocol |
Target preparation was performed according to the standard Affymetrix protocol using the Enzo BioArray RNA transcript labeling kit.
|
| |
|
| Hybridization protocol |
Affimetrix EukGE-WS2 on a GeneChip Fluidics Station 400
|
| Scan protocol |
Arrays were scanned twice using a GeneArray Scanner (Agilent/Affymetrix).
|
| Description |
Monitor the effects of the deletion of the repellent gene rep1 on expression profiles in SG200.
|
| Data processing |
MAS5.0
|
| |
|
| Submission date |
Apr 23, 2010 |
| Last update date |
May 01, 2011 |
| Contact name |
Wieke R. Teertstra |
| E-mail(s) |
w.r.teertstra@uu.nl
|
| Phone |
0031302539472
|
| Fax |
0031302532837
|
| URL |
http://www.uu.nl/EN/faculties/science/organisation/depts/biology/research/chairs/microbiology/research/
|
| Organization name |
University of Utrecht
|
| Department |
Biology
|
| Lab |
Microbiology
|
| Street address |
Padualaan 8
|
| City |
Utrecht |
| ZIP/Postal code |
3584CH |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL3681 |
| Series (1) |
| GSE21490 |
Gene expression in aerial hyphae in response to rep1 deletion in Ustilago maydis |
|
