|
| Status |
Public on Jan 01, 2011 |
| Title |
Muscle_3030_HRT_user |
| Sample type |
RNA |
| |
|
| Source name |
Human skeletal muscle
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
tissue: Vastus lateralis muscle
hrt status: user of postmenopausal, estrogen-based HRT
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent. The RNA/cRNA concentrations were checked with Nanodrop ND-1000 (Thermo Fisher Scientific Inc.) and RNA/cRNA quality using Experion electrophoresis station (Bio-Rad Laboratories).
|
| Label |
biotin
|
| Label protocol |
IVT reaction overnight (14h), during which cRNA was biotinylated (Ambion's Illumina RNA TotalPrep Amplification kit).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were quantile normalised using the affy package for R.
|
| |
|
| Submission date |
Apr 21, 2010 |
| Last update date |
Jan 01, 2011 |
| Contact name |
Paula Ronkainen |
| E-mail(s) |
paula.h.a.ronkainen@jyu.fi
|
| Organization name |
University of Jyväskylä
|
| Street address |
PO Box 35
|
| City |
Jyväskylä |
| ZIP/Postal code |
40014 |
| Country |
Finland |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE21421 |
Global gene expression profiles in skeletal muscle of monozygotic female twins discordant for hormone replacement therapy |
|
