|
| Status |
Public on Apr 20, 2010 |
| Title |
SAPKO_CXCR5_Positive_Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse SAP-/- CXCR5+ CD4 T cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: SAP-/- C57BL6/J Mice
|
| Treatment protocol |
Splenocytes were isolated and antigen-specific CD4 T cells were enriched using anti-CD45.1-FITC and magnetic bead purification (Miltenyi). Virus-specific SMtg CD4+CD45.1+TCRβ+CD19-7AAD- cells were then sorted into TFH and non-TFH populations on the basis of CXCR5 expression using a FACSAria (BD Biosciences).
|
| Growth protocol |
All infections were done by intraperitoneal injection of 1-2 x 10^5 plaque-forming units (PFU) of LCMV Armstrong per mouse. 6,000 naïve WT or Sh2d1a-/- SMtg (SMARTA LCMV-specific TCR transgenic) CD4 T cells were transferred per mouse, prior to LCMV infection. Spleens were harvested 8 days after LCMV infection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Qiagen RNAeasy Mini spin columns (Qiagen), including QiaShredder and on-column digestion of genomic DNA. Some RNA samples were then concentrated using MinElute spin columns (Qiagen).
|
| Label |
biotin
|
| Label protocol |
RNA quality of all samples was confirmed by BioAnalyzer Nano gel (Agilent), then probes were generated from 30ng total RNA by single round linear amplification using the Ovation Pico system (Nugen).
|
| |
|
| Hybridization protocol |
Standard Affymetrix
|
| Scan protocol |
Standard Affymetrix
|
| Description |
Gene expression data from SAP-/- CXCR5+ CD4+ virus specific T follicular helper cells
|
| Data processing |
MAS5
|
| |
|
| Submission date |
Apr 19, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Shane Crotty |
| E-mail(s) |
shane@liai.org
|
| Organization name |
La Jolla Institute for Allergy and Immunology
|
| Department |
Vaccine Discovery
|
| Lab |
VD-Crotty
|
| Street address |
9420 Athena Circle
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE21379 |
Expression Data from WT and Sh2d1a-/- in vivo follicular helper CD4 T cells (TFH) versus non follicular helper CD4 T cells (non-TFH) |
| GSE21381 |
Germinal center T follicular helper cell IL-4 production is dependent on SLAM receptor (CD150) |
|
| Relations |
| Reanalyzed by |
GSE119085 |
