|
| Status |
Public on Apr 24, 2010 |
| Title |
impaired fasting glucose 3_microRNA |
| Sample type |
RNA |
| |
|
| Source name |
blood
|
| Organism |
Homo sapiens |
| Characteristics |
patient type: impaired fasting glucose 3
gender: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Hy3
|
| Label protocol |
LNA-modified oligonucleotide (Exiqon) probes for human, mouse and rat miRNAs annotated in miRBase version 11.0 were used in the microarray. Total RNA (1 μg) was 3’-end –labeled with Cy3 dye using the miRCURY LNA ™ Power Labeling Kit (Exiqon)
|
| |
|
| Hybridization protocol |
hybridized on miRCURY LNA™ Arrays, using MAUI® hybridization system
|
| Scan protocol |
Chips were scanned on Innopsys Scan 700, microarray scanner at 532 nm
|
| Description |
replicate 3
|
| Data processing |
miRNA microarray data were analyzed by subtracting the mean background signal (B532nm) from the test signal (F532nm)
|
| |
|
| Submission date |
Apr 13, 2010 |
| Last update date |
Apr 23, 2010 |
| Contact name |
Kandiah Jeyaseelan |
| E-mail(s) |
erijeya@nus.edu.sg
|
| Organization name |
National University of Singapore
|
| Department |
Biochemistry
|
| Street address |
8 Medical Drive
|
| City |
Singapore |
| ZIP/Postal code |
117597 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL10322 |
| Series (2) |
| GSE21321 |
Blood microRNA profiles and upregulation of hsa-miR-144 in males with type 2 diabetes mellitus. |
| GSE26168 |
Type 2 Diabetes mellitus: mRNA and miRNA profiling |
|
