|
| Status |
Public on Mar 23, 2014 |
| Title |
iPS2 osKM2 |
| Sample type |
RNA |
| |
|
| Source name |
iPS2 osKM2
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human iPS line D2osKM2, grown on MEFs
potency, inductors: Pluripotent, (Oct4, Sox2 from Axolotl)-(KLF4, MYC from Human)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
(Oct4, Sox2 from Axolotl)-(KLF4, MYC from Human) Human iPS line D2osKM2, grown on MEFs
|
| Data processing |
The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina) background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor
|
| |
|
| Submission date |
Apr 12, 2010 |
| Last update date |
Jan 15, 2022 |
| Contact name |
Marcos J. Araúzo-Bravo |
| E-mail(s) |
mararabra@yahoo.co.uk
|
| Phone |
+34 943 00 6108
|
| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Department |
Cell and Developmental Biology
|
| Lab |
Computational Biology and Bionformatics
|
| Street address |
Rogentstrasse
|
| City |
Muenster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE21302 |
Reprogramming to pluripotency is an ancient trait of vertebrate Oct4 and Pou2 proteins |
|
