|
| Status |
Public on May 12, 2021 |
| Title |
24h after treatment rep 2 |
| Sample type |
SRA |
| |
|
| Source name |
U87MG cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U87MG
treatment: enzalutamide for 24hr
|
| Treatment protocol |
Cells were treated with enzalutamide for different time points before isolating the total RNA from the cells.
|
| Growth protocol |
U87MG cells are grown in DMEM medium supplemented with 10% FBS and 1% P/S. The cells are split every 3-4 days and change the medium every 2 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were harvested after differentiation and total RNA was isolated with RNeasy isolation kit (Qiagen) according to manufacturer’s instructions. On-column DNase treatment was performed to remove genomic DNA contamination (Qiagen).
Libraries were prepared according to Illumina's instructions accompanying the RNA Sample Kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
mRNA
|
| Data processing |
Illumina software used for basecalling.
Sequenced reads were trimmed for adaptor sequence then mapped to human genome using bowtie.
Reads counts for all genes.
Genome_build: Human genome hg19 (GRCh37)
Supplementary_files_format_and_content: Tab-delimited plain text files: the first column is gene IDs and the second column is reads counts.
|
| |
|
| Submission date |
May 11, 2021 |
| Last update date |
May 14, 2021 |
| Contact name |
Chi Zhang |
| E-mail(s) |
czhang5@unl.edu
|
| Organization name |
University of Nebraska - Lincoln
|
| Street address |
1901 Vine Street
|
| City |
Lincoln |
| State/province |
NE |
| ZIP/Postal code |
68588 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE174295 |
Androgen receptor, although not a specific marker for, is a novel target to suppress glioma stem cells as a therapeutic strategy for glioblastoma |
|
| Relations |
| BioSample |
SAMN19111518 |
| SRA |
SRX10863400 |
