|
| Status |
Public on Jun 30, 2010 |
| Title |
endometrium, day 8, pregnant, mare#2 |
| Sample type |
RNA |
| |
|
| Source name |
endometrium, day 08, pregnant
|
| Organism |
Equus caballus |
| Characteristics |
breed: warmblood
mare#: 2
pregnancy: pregnant
tissue: endometrial tissue
time: 8 d
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the 12 endometrial biopsy samples using Trizol® Reagent (Invitrogen GmbH, Karlsruhe, Germany) according to the manufacturer’s instructions. Quantity and purity of RNA were measured with a NanoDrop 1000 (PEQLAB Biotechnologie GMBH, Erlangen, Germany). Quality of total RNA was determined by electrophoresis using an Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany). RNA integrity (RIN) values ranged from 8.3 to 9.2.
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled cRNA was produced using the Quick Amp Labeling Kit, one-color (Agilent Technologies) according the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Microarray analysis was performed using Agilent 4x44k Horse Gene Expression microarrays (AMADID 021322). Labeled cRNA was hybridized to the microarrays according the manufacturer’s instructions.
|
| Scan protocol |
Hybridized and washed slides were scanned at 3 µm resolution with an Agilent DNA Microarray Scanner (G2505C, Agilent Technologies).
|
| Description |
endometrial tissue sample collected from day 08 pregnant mare after embryo recovery
|
| Data processing |
Image processing was performed with Feature Extraction Software 10.5.1.1 (Agilent Technologies). Processed signals were filtered based on “Well above background” flags (detection in 4 of 6 samples in either one of the two experimental groups) and subsequently normalized with the BioConductor package vsn.
|
| |
|
| Submission date |
Mar 24, 2010 |
| Last update date |
Mar 24, 2010 |
| Contact name |
Stefan Michael Bauersachs |
| E-mail(s) |
stefan.bauersachs@uzh.ch
|
| Organization name |
University of Zurich
|
| Department |
Department for Farm Animals
|
| Lab |
Genetics and Functional Genomics
|
| Street address |
Eschikon 27 EHB 23.1
|
| City |
Lindau |
| State/province |
Zurich |
| ZIP/Postal code |
8315 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL10248 |
| Series (1) |
| GSE21046 |
Identification of differentially expressed genes in equine endometrium at day 8 and 12 of pregnancy |
|
