|
| Status |
Public on Aug 27, 2010 |
| Title |
GIST_04_KIT (expression) |
| Sample type |
RNA |
| |
|
| Source name |
GIST tumor, KIT mutated
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: gastrointestinal stromal tumor
gender: M
age: 79
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed with RNeasy spin columns on flash frozen tumor specimens, according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 7G.
|
| Description |
Gene expression data from GIST with KIT mutation
|
| Data processing |
The data were analyzed with Bioconductor affy package, using RMA algorithm for normalization and summarization.
|
| |
|
| Submission date |
Mar 09, 2010 |
| Last update date |
Aug 27, 2010 |
| Contact name |
Maria Abbondanza Pantaleo |
| E-mail(s) |
maria.pantaleo@unibo.it
|
| Organization name |
Policlinico S. Orsola-Malpighi, University of Bologna
|
| Department |
Institute of Hematology and Medical Oncology "L.A.Seragnoli"
|
| Street address |
via Massarenti 9
|
| City |
Bologna |
| State/province |
BO |
| ZIP/Postal code |
40138 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE20708 |
Gene expression data from GIST with KIT mutation |
| GSE20710 |
Integrative analysis of gene expression profiling and genomic copy number in Gastrointestinal Stromal Tumors |
|
