|
| Status |
Public on Jun 16, 2010 |
| Title |
042409_Rainey_Yewei_HFA-48h-ACTH_Sample_Probe_Profile |
| Sample type |
RNA |
| |
|
| Source name |
human primary fetal adrenal cells
|
| Organism |
Homo sapiens |
| Characteristics |
agent: ACTH
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN Rneasy plus mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
triplicate 2 (ACTH)
|
| Data processing |
The raw data is generated through GenomeStudio software. The raw data were normalised using quantile normalisation with IlluminaGUI in R. Genespring 11 software was used for subsequent data analysis.
|
| |
|
| Submission date |
Mar 08, 2010 |
| Last update date |
Mar 08, 2010 |
| Contact name |
yewei xing |
| E-mail(s) |
yxing@mcg.edu
|
| Phone |
7067218779
|
| Fax |
7067218360
|
| Organization name |
Medical College of Georgia
|
| Department |
Physiology
|
| Street address |
1120 15th street
|
| City |
Augusta |
| State/province |
GA |
| ZIP/Postal code |
30912 |
| Country |
USA |
| |
|
| Platform ID |
GPL6883 |
| Series (2) |
| GSE20675 |
Genome-wide analysis of fetal adrenocortical cells gene expression after 48 h ACTH treatment |
| GSE20676 |
Genome-wide analysis of adrenocortical cells gene expression after 48 h ACTH treatment |
|
