|
| Status |
Public on Jun 02, 2010 |
| Title |
HeLa_Control (Cy3)_HeLa_MALAT-1 (Cy5)_Replication 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
HeLa Control Cy3
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa TO cells
protocol: control
|
| Treatment protocol |
Control
|
| Growth protocol |
Cells were grown at 37°C, 5% CO2 in Dulbecco’s Modified Eagle's Medium supplemented with 10% fetal calf serum and penicillin/streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Invitrogen Trizol reagent
|
| Label |
Cy3
|
| Label protocol |
Low RNA Input Linear Amplification kit standard protocol
|
| |
|
| Channel 2 |
| Source name |
HeLa MALAT Cy5
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa TO cells
protocol: MALAT-1 knockdown
sirna: siMALAT2
|
| Treatment protocol |
To carry out RNA interference, siRNA duplexes, synthesized by SIGMA or Invitrogen were transfected into cells using Lipofectamine RNAi MAX (Invitrogen) according to the manufacturer's guidelines
|
| Growth protocol |
Cells were grown at 37°C, 5% CO2 in Dulbecco’s Modified Eagle's Medium supplemented with 10% fetal calf serum and penicillin/streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Invitrogen Trizol reagent
|
| Label |
Cy5
|
| Label protocol |
Low RNA Input Linear Amplification kit standard protocol
|
| |
|
| |
| Hybridization protocol |
Hybridization_Kit: Agilent in situ hybridization plus
|
| Scan protocol |
Scanner: Agilent DNA microarray Scaner
|
| Description |
Mammalian non-coding transcript
Hela Malat 100-1
|
| Data processing |
Agilent Feature Extraction ver 9.5, normalized (lowess and rank consistency)
|
| |
|
| Submission date |
Feb 24, 2010 |
| Last update date |
Jun 01, 2010 |
| Contact name |
RANDEEP RAKWAL |
| E-mail(s) |
plantproteomics@gmail.com
|
| Phone |
+81-(0)90-1853-7875
|
| Organization name |
University of Tsukuba
|
| Department |
Institute of Health and Sport Sciences
|
| Lab |
Global Sport Innovation (GSI) 403
|
| Street address |
1-1-1 Tennodai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8574 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE20506 |
Identification of cis- and trans-acting factors involved in the localization of MALAT-1 to nuclear speckles |
|
