|
| Status |
Public on Aug 15, 2021 |
| Title |
MGHIPIPD1001_092414_bam |
| Sample type |
SRA |
| |
|
| Source name |
frozen or formalin-fixed tumor
|
| Organism |
Homo sapiens |
| Characteristics |
treatment state: ON
timepoint (days): 153
antibody: anti-PD1+anti-CTLA4
response immune checkpoint blockade therapy: NR
age at the baseline: 21
batch: 2
tumor type: metastatic melanoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified from patients' frozen or formalin-fixed tumor specimens using the TRIzol protocol. Illumina TruSeq RNA Sample PRE Immune Checkpoint Blockade Therapyp Kit (Cat#FC-122-1001) was used with 250 ng of total RNA for the construction of sequencing libraries.
RNA libraries were PRE Immune Checkpoint Blockade Therapypared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
MGH Patient IPIPD1001
|
| Data processing |
summary read counts by featureCounts v1.5.1
Genome_build: hg19
Supplementary_files_format_and_content: csv of the read counts matrix generated by featureCounts
|
| |
|
| Submission date |
Mar 03, 2021 |
| Last update date |
Aug 15, 2021 |
| Contact name |
Tian Tian |
| E-mail(s) |
tt72@njit.edu
|
| Organization name |
Children's Hospital of Philadelphia
|
| Department |
Center of Applied Genomics
|
| Street address |
3615 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL18573 |
| Series (1) |
| GSE168204 |
Pathway Signatures Derived from On-treatment Tumor Specimens Predict Response to Anti-PD1 Blockade in Metastatic Melanoma |
|
| Relations |
| BioSample |
SAMN18133110 |
| SRA |
SRX10234755 |
