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Sample GSM5123974 Query DataSets for GSM5123974
Status Public on Sep 29, 2021
Title HT9L: Tibetan placenta mRNA
Sample type RNA
Source name placenta
Organism Homo sapiens
Characteristics gender: Female
tissue: Placenta
population: Tibetan
Extracted molecule total RNA
Extraction protocol Total RNA from placentas was isolated using RNeasy Mini Kits according to the manufacturer’s instructions. RNA quantity was determined using an Agilent Bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol cRNA in hybridization solution was denatured at 99℃ for 5 min and 45℃ for 5 min prior to loading onto a microarray. Arrays were hybridized in a Agilent Hybridization Oven for 16hr at a rotation speed of 60 rpm at 45℃ and washed with Wash Buffer A and stained in the Affymetrix Fluidics Station 450.
Scan protocol Scanned on Affymetrix GeneChip Scanner 3000
Description Gene expression data from Tibetan placenta
Data processing AGCC Software (Affymetrix® GeneChip® Command Console® Software) converts.DAT files from image signals to digital signals known as.CEL files. CEL files record probe level fluorescent signals.
Submission date Mar 02, 2021
Last update date Sep 29, 2021
Contact name Tanna Wuren
Organization name Qinghai University
Street address Kunlun Road No.16
City xining
ZIP/Postal code 810001
Country China
Platform ID GPL15207
Series (2)
GSE168021 Gene expression in Placental Tissue from high altitude European and Tibetan women
GSE168130 miRNA and mRNA expression in placental tissue from high altitude pregnant women

Data table header descriptions
VALUE Values presented are RMA signal intensity.

Data table
AFFX-BioB-5_at 145.4188
AFFX-BioB-M_at 196.5329
AFFX-BioB-3_at 147.8688
AFFX-BioC-5_at 364.7806
AFFX-BioC-3_at 523.6470
AFFX-BioDn-5_at 1059.9360
AFFX-BioDn-3_at 2274.9170
AFFX-CreX-5_at 5723.6710
AFFX-CreX-3_at 6911.3700
AFFX-DapX-5_at 22.8476
AFFX-DapX-M_at 171.8432
AFFX-DapX-3_at 557.0548
AFFX-LysX-5_at 23.3894
AFFX-LysX-M_at 53.8916
AFFX-LysX-3_at 109.8202
AFFX-PheX-5_at 44.2363
AFFX-PheX-M_at 46.5384
AFFX-PheX-3_at 84.6419
AFFX-ThrX-5_at 10.1562
AFFX-ThrX-M_at 38.2174

Total number of rows: 49395

Table truncated, full table size 1043 Kbytes.

Supplementary file Size Download File type/resource
GSM5123974_150106-140651B_HT9L_PrimeView.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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